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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

FeFAB assay indicated that the culture supernatants collected from the infected and drug-pretreated cells contained infectious FFV virions, whichfefab induce expression of the β-galactosidase in the ...

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